%0 Journal Article
%T Antiamoebic activity of marine sponge Haliclona exigua (Krikpatrick)
%A V Lakshmi
%A A. Saxena
%A S.K. Mishra
%A M. Mishra
%J Bangladesh Journal of Pharmacology
%D 2009
%I Bangladesh Pharmacological Society
%X The methanol and methanol-chloroform (1:1) extracts of the freshly collected Haliclona exigua showed minumim inhibitory concentration (MIC) of 125 ¦Ìg/ml and 250 ¦Ìg/ml respectively in in vitro studies, but when both of these were tested in vivo in rats, only methanol-chloroform showed 80% inhibition of trophozoites at the dose of 900 mg/kg body weight against Entamoeba histolytica. Therefore only methanol-chloroform extract was further fractionated into four fractions (hexane, chloroform, n-butanol soluble and n-butanol insoluble fractions). Out of these, only hexane and n-butanol soluble fractions showed 80% inhibition of trophozoites at 900 mg/kg dose. Further the chromatography of the n-butanol fraction yielded araguspongin-C which showed promising results at different doses.
%K Alkaloid
%K Anti-amoebic activity
%K Araguspongin-C
%K Haliclona exigua
%K Marine sponge
%U http://www.bdjpharmacol.com/0401/55.pdf