%0 Journal Article
%T Chlorophyllin inhibits the multiplication of poliovirus in hep-2 cell cultures Clorofilina inibe a multiplica  o de poliov¨ªrus em cultura de c¨¦lulas hep-2
%A L¨ªgia Carla Faccin
%A Jos¨¦ Augusto Ramos Medeiros
%A Mario Sergio Mantovani
%A Carlos Nozawa
%J Semina : Ci¨ºncias Biol¨®gicas e da Sa¨²de
%D 2008
%I Universidade Estadual de Londrina
%X Chlorophyllin (CHLN), a semisynthetic derivative of chlorophyll with antimutagenic properties, was assayed for its antiviral effect on the replication of poliovirus in cell culture. The drug was tested for the virucide, prophylactic and therapeutic activities on the replication of the poliovirus in HEp-2 cells cultures, at concentrations of 0.5 and 2.5 ¦Ìg/mL. Virus titration and an indirect immunofluorescence test were used for the evaluation. The CHLN inhibited poliovirus replication in all treatment protocols; however, it was more effective on virucide treatment, with a 95.7% reduction in viral multiplication at concentration of 2.5 ¦Ìg/mL. CHLN reduced the number of specifically fluorescent infected cells in both virucide and therapeutic treatments, 8h and 10h post-infection, at both concentrations (0.5 and 2.5 ¦Ìg/ mL). It is suggested that CHLN either has a direct action on the virus particles or acts on the initial stage of the poliovirus replication. A clorofilina (CHLN), derivado sint¨¦tico da clorofila com atividade antimutag¨ºnica, foi analisada quantoa sua atividade antiviral para o poliov¨ªrus em culturas de c¨¦lulas HEp-2. A droga foi avaliada quanto ¨¤s atividades virucida, profil¨¢tica e terap¨ºutica na multiplica  o do poliovirus em culturas de c¨¦lulas HEp-2, nas concentra  es de 0,5 e 2,5 ¦Ìg/mL. A inibi  o do t¨ªtulo viral e a rea  o de imunofluoresc¨ºncia foram utilizadas para avalia  o. A CHLN inibiu a multiplica  o do poliov¨ªrus nos tr¨ºs protocolos de tratamento, por¨¦m foi mais efetiva no tratamento virucida inibindo em 95,7% a multiplica  o viral na concentra  o de 2,5 ¦Ìg/mL. A CHLN reduziu o n¨²mero de c¨¦lulas infectadas com fluoresc¨ºncia espec¨ªfica, nos tratamentos virucida, e terap¨ºutico, 8h e 10h p¨®s-infec  o, nas duas concentra  es (0,5 e 2,5 ¦Ìg/mL) . Sugere-se que a CHLN tem uma a  o direta na part¨ªcula viral ou provavelmente age nos est¨¢gios iniciais da multiplica  o do poliovirus.
%K Clorofilina
%K Poliov¨ªrus
%K Antiviral
%K Cultura de c¨¦lulas.
%U http://www.uel.br/revistas/uel/index.php/seminabio/article/view/3455