%0 Journal Article
%T In vivo evaluation of anticlastogenicity of extracts from medicinal plants of Miconia genus using the micronucleus test Avalia  o in vivo da anticlastogenicidade de extratos de plantas medicinais do g那nero Miconia atrav谷s do teste do micron迆cleo
%A Juliana Mara Serpeloni
%A Wagner Vilegas
%A Eliana A. Varanda
%A Ilce Mara S. C車lus
%J Semina : Ci那ncias Biol車gicas e da Sa迆de
%D 2008
%I Universidade Estadual de Londrina
%X The genus Miconia is comprised of approximately 1000 species. For some of them, biological activities have already been described such as the analgesic and the anti-microbial ones. The purpose of this work was to evaluate the possible protective and cytotoxic effects of the methanolic extract from M. albicans, M. cabucu, M. rubiginosa and M. stenostachya and the chloroformic extract from M. albicans in mice bone marrow cells in 540 mg/kg p.c. dose. The extracts were administered by means of forced feeding and the cyclophosphamide (CPA) was applied intraperitonially one hour after supplementation with extracts. All animals were submitted to euthanasia 30 hours after the treatment. The analyzed cells were extracted from mice bone marrow according to protocol described by Schmid (1975). The cytotoxicity of the extracts was evaluated through the percentage of polychromatic erythrocytes (PCE) in 200 erythrocytes (PCE + NCE). Two thousand PCEs of each animal were analyzed and the micronucleated polychromatic erythrocytes (MNPCEs) frequencies were scored. The results obtained indicated that none of the extracts associated with the CPA showed cytotoxic effect and only the extracts of M. rubiginosa and M. stenostachya showed protective effect to DNA. Chemical analyses of the extracts showed that the four species studied contain mainly flavonoids, phenolic compounds and tannins. The phytochemical characterization of these extracts could contribute to elucidate the protective effect presented only by the species M. rubiginosa and M. stenostachya, besides making it possible the study of other therapeutic activities. O g那nero Miconia possui aproximadamente 1000 esp谷cies, e para algumas, j芍 foram descritas atividades biol車gicas como a analg谷sica e antimicrobiana. Esse trabalho teve como objetivo avaliar os poss赤veis efeitos protetores e citot車xicos dos extratos metan車licos de M. albicans, M. cabucu, M. rubiginosa e M. stenostachya e do extrato clorof車rmico de M. albicans em c谷lulas da medula 車ssea de camundongos na dose de 540 mg/kg p.c. Os extratos foram administrados via gavage e a ciclofosfamida (CPA) foi aplicada intraperitonealmente 1h, ap車s a suplementa  o com os extratos. Todos os animais foram submetidos 角 eutan芍sia 30h ap車s o tratamento. As c谷lulas analisadas foram retiradas da medula 車ssea de acordo com protocolo descrito por Schmid (1975). A citotoxicidade dos extratos foi avaliada pela percentagem de eritr車citos policrom芍ticos (PCE) em 200 eritr車citos (PCE + NCE). Foram analisados 2000 PCEs por animal e anotadas as freq邦那ncias de MNPCEs. Os resultados obt
%K Micron迆cleo
%K Plantas medicinais
%K Anticlastogenicidade.
%U http://www.uel.br/revistas/uel/index.php/seminabio/article/view/3452