%0 Journal Article
%T Immunohistochemical characterization of neoplasms of the urinary bladder associated with bovine enzootic hematuria Caracteriza  o imunoistoqu赤mica de neoplasias de bexigas associadas 角 hemat迆ria enzo車tica bovina
%A Maria Aparecida da Silva
%A Julio Lopes Sequeira
%A Ana Paula Madureira
%A Louisiane Carvalho Nunes
%J Semina : Ci那ncias Agr芍rias
%D 2013
%I Universidade Estadual de Londrina
%X Bovine enzootic hematuria is characterized by the development of hemangiomatous lesions from several types of neoplastic processes, from epithelial and mesenchymal origin. In this research the histogenesis of neoplastic lesions found in bladder of bovines with enzootic hematuria from Capara車 microregion in the state of Esp赤rito Santo, Brazil was determined. To accomplish this objective, immunohistochemical analysis was performed with primary antibodies: anti-vimentin, anti-cytokeratin, anti-CD31 and anti-uroplakin. Neoplasms found included urothelial carcinoma, in situ carcinoma, adenocarcinoma, hemangioma, myxoma e hemangiosarcoma. Immunohistochemical staining of cytokeratin in epithelial neoplasms and vimentin in mesenchymal neoplasms was significant (p<0.05). CD31 was positive in all the vessels of all samples, however, the staining was significant (p<0.05) in the tumor endothelial cells of the vascular mesenchymal neoplasms, as in hemangiomas and hemangiosarcomas. Uroplakin III staining was uneven in several neoplastic types and showed no significant difference (p>0.05). Most neoplasms showed an atypical uroplakin staining on urothelium and, in the case of hemangiosarcomas there was no staining of the urothelium at all. The Spearman statistical analysis revealed a positive correlation (r= 0.63, p= 0.05) between CD31 and vimentin and between cytokeratin and uroplakin (rs= 每 0.61, p= 0.05). It was concluded that biomarkers anti-cytokeratin, anti-vimentin and anti-CD31 are important for the diagnosis of neoplasms epithelial, mesenchymal and vascular mesenchymal, respectively. It is possible to use vimentin and CD31 in association in vascular mesenchymal neoplasms and cytokeratin and uroplakin in epithelial neoplasms. The uroplakin is an effective marker, not only for tumor diagnosis, but also to evaluate the urothelial integrity. A hemat迆ria enzo車tica bovina 谷 caracterizada pelo desenvolvimento de les es hemangiomatosas relacionadas a diversos tipos de processos neopl芍sicos de origem epitelial e mesenquimal. O uso de biomarcadores pode auxiliar na classifica  o tumoral. Objetivou-se com este trabalho determinar a histog那nese das les es neopl芍sicas encontradas em bexigas, de animais da microrregi o do Capara車, ES com hemat迆ria enzo車tica bovina. Para isto foi realizada a imunoistoqu赤mica com os anticorpos prim芍rios: anti-vimentina, anti-citoqueratina, anti-CD31 e anti-uroplaquina. As neoplasias encontradas foram carcinoma urotelial, carcinoma in situ, adenocarcinoma, hemangioma, mixoma e hemangiossarcoma. As marca  es da citoqueratina em neoplasias epitel
%K Citoqueratina
%K Vimentina
%K CD31
%K Uroplaquina III
%K Hemat迆ria enzo車tica bovina.
%U http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/11062