%0 Journal Article
%T Adaptation of a visualized loop-mediated isothermal amplification technique for field detection of Plasmodium vivax infection
%A Zhi-Yong Tao
%A Hua-Yun Zhou
%A Hui Xia
%A Sui Xu
%A Han-Wu Zhu
%A Richard L Culleton
%A Eun-Taek Han
%A Feng Lu
%A Qiang Fang
%A Ya-Ping Gu
%A Yao-Bao Liu
%A Guo-Ding Zhu
%A Wei-Ming Wang
%A Ju-Lin Li
%A Jun Cao
%A Qi Gao
%J Parasites & Vectors
%D 2011
%I BioMed Central
%R 10.1186/1756-3305-4-115
%X A visualized LAMP method was established by the addition of a microcrystalline wax-dye capsule containing the highly sensitive DNA fluorescence dye SYBR Green I to a normal LAMP reaction prior to the initiation of the reaction. A total of 89 blood samples were collected on filter paper and processed using a simple boiling method for DNA extraction, and then tested by the visualized LAMP method for Plasmodium vivax infection.The wax capsule remained intact during isothermal amplification, and released the DNA dye to the reaction mixture only when the temperature was raised to the melting point following amplification. Soon after cooling down, the solidified wax sealed the reaction mix at the bottom of the tube, thus minimizing the risk of aerosol contamination. Compared to microscopy, the sensitivity and specificity of LAMP were 98.3% (95% confidence interval (CI): 91.1-99.7%) and 100% (95% CI: 88.3-100%), and were in close agreement with a nested polymerase chain reaction method.This novel, cheap and quick visualized LAMP method is feasible for malaria diagnosis in resource-limited field settings.Between the years 2000 and 2008, there was re-emergence of Plasmodium vivax malaria in the central part of the People's Republic of China (P. R. China) [1]. Rapid and accurate diagnosis of malaria is not only crucial for patient treatment, but also important for disease control, especially during attempts at elimination, as P. vivax infections are often found at low parasite densities, and any missed cases of malaria could be a potential source of local transmission. Microscopic examination of blood films is the most wildly used diagnostic approach in the field and still remains the 'gold' standard. However, this method is labour-intensive, requires well-trained experts and may result in therapeutic delays. Recently developed lateral flow-based malaria rapid diagnostic tests (RDTs) have proved useful in P. falciparum-endemic countries, as the sensitivity of RDTs against P.
%U http://www.parasitesandvectors.com/content/4/1/115