%0 Journal Article
%T Evaluation and In-House Validation of Five DNA Extraction Methods for PCR-based STR Analysis of Bloodstained Denims
%A Gayvelline Calacal
%A Henry Perdigon
%A Kristine Co Seng
%A Maria Corazon De Ungria
%A Saturnina Halos
%J -
%D 2004
%X One type of crime scene evidence commonly submitted for analysis is bloodstain on denim. However, chemicals (e.g., indigo) used to produce denim materials may co-purify with DNA and hence, affect subsequent DNA analysis. The present study compared five methods (e.g., standard organic, organic with hydrogen peroxide (H2O2), modified FTA£¿, organic/Chelex£¿-Centricon£¿, and QIAamp£¿ DNA Mini Kit-based procedures) for the isolation of blood DNA from denim. A Short Tandem Repeat (STR)-based analysis across two to nine STR markers, namely, HUMvWA, HUMTH01, D8S306, HUMFES/FPS, HUMDHFRP2, HUMF13A01, HUMFGA, HUMTPOX, and HUMCSF1PO, was used to evaluate successful amplification of blood DNA extracted from light indigo, dark indigo, indigo-sulfur, pure indigo, sulfur-top, and sulfur-bottom denim materials. The results of the present study support the utility of organic/Chelex£¿-Centricon£¿ and QIAamp£¿ Kit procedures in extracting PCR-amplifiable DNA from five different types of denim materials for STR analysis. Furthermore, a solid-based method using FTA£¿ classic cards was modified to provide a simple, rapid, safe, and cost-effective procedure for extracting blood DNA from light, dark indigo and pure indigo denim materials. However, DNA eluted from bloodstained sulfur-dyed denims (e.g., sulfur-top and sulfur-bottom) using FTA£¿ procedure was not readily amplifiable
%U http://journals.upd.edu.ph/index.php/sciencediliman/article/view/102