%0 Journal Article
%T Non-association of IL-12 +1188 and IFN-¦Ã +874 polymorphisms with cytokines serum level in occult HBV infected patients
%A Arababadi Mohammad
%A Pourfathollah Ali
%A Jafarzadeh Abdollah
%A Hassanshahi Gholamhossein
%J Saudi Journal of Gastroenterology
%D 2011
%I Medknow Publications
%X Background/Aim: Occult hepatitis B infection (OBI) is identified as a form of hepatitis in which despite the absence of detectable HBsAg, HBV-DNA is observed in peripheral blood of patients. The main aim of this study has been to investigate the association between polymorphisms in +874 of IFN-¦Ã and +1188 of IL-12 with their serum level in patients suffering from OBI. Materials and Methods: In this experimental study, plasma samples of 3700 blood donors were tested for the presence of hepatitis B surface antigen (HBsAg) and anti-HBc by ELISA. The HBsAg - /anti-HBc + samples were selected and screened for HBV-DNA by PCR. HBV-DNA positive samples were assigned as OBI cases and ARMS-PCR techniques were performed to examine the two known polymorphisms within IL-12 and IFN-¦Ã. In addition, the serum levels of IL-12 and IFN-¦Ã were also determined by ELISA. Results: Results of this study demonstrated that, 352 (9.5%) out of 3700 blood samples were HBsAg - /anti-HBc + and HBV-DNA was detected in 57/352 (16.1%) of HBsAg - /anti-HBc + samples. Our results showed that groups showed significant difference in CC allele of +1188 region of IL-12 and no difference was observed in the other evaluated genes. Our results also showed that the alleles of +1188 region of IL-12 and alleles of +874 of IFN-¦Ã were also not associated with serum level of cytokines. Conclusion: According to the results of this study, it may be concluded that the polymorphisms in +1188 region of IL-12 and +874 region of IFN-¦Ã would not affect the expression of both cytokines at serum level in OBI patients.
%K IL-12
%K IFN-¦Ã
%K occult hepatitis B infection
%K polymorphism
%U http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2011;volume=17;issue=1;spage=30;epage=35;aulast=Arababadi