%0 Journal Article
%T Pronase-free B-cell flow-cytometry crossmatch
%A Hajeer A
%A Saleh S
%A Sutton P
%A Shubaili A
%J Saudi Journal of Kidney Diseases and Transplantation
%D 2009
%I Medknow Publications
%X Detection of anti-class II antibodies by panel response assay (PRA) and flow cross-match techniques carries an important value in terms of graft function. Even low levels of pre-formed alloantibodies to HLA class II antigens represent a risk of rejection. We present here a method for blocking non-specific flow crossmatch reactions using pooled, heat-inactivated rabbit serum. This method shows very low background and minimal non-specific reactions. In addition, it avoids the use pronase enzyme that can non-specifically digest different cell surface proteins.
%U http://www.sjkdt.org/article.asp?issn=1319-2442;year=2009;volume=20;issue=4;spage=662;epage=665;aulast=Hajeer