%0 Journal Article
%T Lamina I NK1 expressing projection neurones are functional in early postnatal rats and contribute to the setting up of adult mechanical sensory thresholds
%A Sharon HW Man
%A Sandrine M Geranton
%A Stephen P Hunt
%J Molecular Pain
%D 2012
%I BioMed Central
%R 10.1186/1744-8069-8-35
%X At P3, a subset of lamina I neurons were found to express the neurokinin 1 (NK1) receptor. Using fluorogold retrograde tracing, we found that the NK1 positive neurons projected upon the parabrachial nucleus (PB) within the hindbrain. Using c-fos immunohistochemistry, we showed that lamina I and PB neurons in P3 rats responded to noxious stimulation of the periphery. Finally, ablation of lamina I neurons with substance-P saporin conjugates at P3 resulted in increased mechanical sensitivity from P45 onwards compared to control animals of the same age.These results suggest that the lamina I pathway is present and functional at least from P3 and required for establishing and fine-tuning mechanical sensitivity in adult rats.
%K Lamina 1
%K Projection neurones
%K Parabrachial nucleus
%K c-fos
%K NK1
%K Formalin
%K Postnatal rat
%K Substance-P saporin
%K Mechanical sensory thresholds
%U http://www.molecularpain.com/content/8/1/35/abstract