%0 Journal Article
%T Anaplastic oligodendrogliomas with 1p19q codeletion have a proneural gene expression profile
%A Fran£¿ois Ducray
%A Ahmed Idbaih
%A Aur¨¦lien de Reyni¨¨s
%A Ivan Bi¨¨che
%A Jo£¿lle Thillet
%A Karima Mokhtari
%A S¨¦verine Lair
%A Yannick Marie
%A Sophie Paris
%A Michel Vidaud
%A Kh¨º Hoang-Xuan
%A Olivier Delattre
%A Jean-Yves Delattre
%A Marc Sanson
%J Molecular Cancer
%D 2008
%I BioMed Central
%R 10.1186/1476-4598-7-41
%X The two groups of gliomas exhibited very different gene expression profiles and were consistently distinguished by unsupervised clustering analysis. One of the most striking differences was the expression of normal brain genes by oligodendrogliomas with 1p19q codeletion. These gliomas harbored a gene expression profile that partially resembled the gene expression of normal brain samples, whereas gliomas with EGFR amplification expressed many genes in common with glioblastoma cancer stem cells. The differences between the two types of gliomas and the expression of neuronal genes in gliomas with 1p19q codeletion were both validated in an independent series of 16 gliomas using real-time RT-PCR with a set of 22 genes differentiating the two groups of gliomas (AKR1C3, ATOH8, BMP2, C20orf42, CCNB1, CDK2, CHI3L1, CTTNBP2, DCX, EGFR, GALNT13, GBP1, IGFBP2, IQGAP1, L1CAM, NCAM1, NOG, OLIG2, PDPN, PLAT, POSTN, RNF135). Immunohistochemical study of the most differentially expressed neuronal gene, alpha-internexin, clearly differentiated the two groups of gliomas, with 1p19q codeletion gliomas showing specific staining in tumor cells.These findings provide evidence for neuronal differentiation in oligodendrogliomas with 1p19q codeletion and support the hypothesis that the cell of origin for gliomas with 1p19q codeletion could be a bi-potential progenitor cell, able to give rise to both neurons and oligodendrocytes.The 1p19q codeletion and EGFR amplification are mutually exclusive and related to dramatically different outcomes in high grade gliomas. The 1p19q codeletion is strongly associated with an oligodendroglial phenotype and favorable prognosis [1]. It has recently been shown to be mediated by a specific t(1;19)(q10;p10) translocation [2]. To date the efforts performed to identify the genes specifically involved in the breakpoint have failed, mostly because both 1p and 19q centromeric regions contain highly repeated sequences. As a consequence the molecular mechanisms unde
%U http://www.molecular-cancer.com/content/7/1/41