%0 Journal Article
%T Molecular and cytological features of the mouse B-cell lymphoma line iMycE¦Ì-1
%A Seong Su Han
%A Arthur L Shaffer
%A Liangping Peng
%A Seung Chung
%A Jae Lim
%A Sungho Maeng
%A Joong Su Kim
%A Nicole McNeil
%A Thomas Ried
%A Louis Staudt
%A Siegfried Janz
%J Molecular Cancer
%D 2005
%I BioMed Central
%R 10.1186/1476-4598-4-40
%X The morphological features and the surface marker expression profile of the iMycE¦Ì-1 cells were evaluated using cytological methods and FACS, respectively. The cytogenetic make-up of the iMycE¦Ì-1 cells was assessed by spectral karyotyping (SKY). The expression of the inserted MycHis gene was determined using RT-PCR and qPCR. Clonotypic immunoglobulin gene arrangements were detected by Southern blotting. The global gene expression program of the iMycE¦Ì-1 cells and the expression of 768 "pathway" genes were determined with the help of the Mouse Lymphochip£¿ and Superarray£¿ cDNA micro- and macroarrays, respectively. Array results were verified, in part, by RT-PCR and qPCR.Consistent with their derivation from LBL, the iMycE¦Ì-1 cells were found to be neoplastic IgMhighIgDlow lymphoblasts that expressed typical B-cell surface markers including CD40, CD54 (ICAM-1), CD80 (B7-1) and CD86 (B7-2). The iMycE¦Ì-1 cells harbored a reciprocal T(9;11) and three non-reciprocal chromosomal translocations, over-expressed MycHis at the expense of normal Myc, and exhibited gene expression changes on Mouse Lymphochip£¿ microarrays that were consistent with MycHis-driven B-cell neoplasia. Upon comparison to normal B cells using eight different Superarray£¿ cDNA macroarrays, the iMycE¦Ì-1 cells showed the highest number of changes on the NF¦ÊB array.The iMycE¦Ì-1 cells may provide a uniquely useful model system to study the growth and survival requirements of Myc-driven mouse LBL in vitro.Gene-targeted iMycE¦Ì mice contain a single-copy mouse MycHis (c-myc) cDNA that has been inserted in opposite transcriptional orientation in the mouse immunoglobulin heavy-chain gene cluster, Igh. The specific insertion site of the MycHis transgene is in the intervening region of the Igh joining gene locus, JH, and the intronic heavy-chain enhancer, E¦Ì. The inserted transgene encodes a C-terminal His6 tag that is useful to distinguish message and protein encoded by MycHis and normal Myc [1]. The iMycE¦Ì mice prov
%U http://www.molecular-cancer.com/content/4/1/40