%0 Journal Article
%T Tumor cell behaviour modulation by mesenchymal stromal cells
%A Lucia Kucerova
%A Miroslava Matuskova
%A Kristina Hlubinova
%A Veronika Altanerova
%A Cestmir Altaner
%J Molecular Cancer
%D 2010
%I BioMed Central
%R 10.1186/1476-4598-9-129
%X Here we have demonstrated tumor-promoting effect of AT-MSC on human melanoma A375 cells. AT-MSC coinjection mediated abrogation of tumor latency and supported subcutaneous xenotransplant growth from very low melanoma cell doses. Tumor incidence was also significantly increased by AT-MSC-derived soluble factors. AT-MSC supported proliferation, suppressed apoptosis and modulated melanoma cell responses to cytotoxic drugs in vitro. Expression and multiplex cytokine assays confirmed synergistic increase in VEGF that contributed to the AT-MSC-mediated support of A375 xenotransplant growth. Production of G-CSF and other factors implicated in formation of supportive proinflammatory tumor cell microenvironment was also confirmed. SDF-1¦Á/CXCR4 signalling contributed to tumor-promoting effect of systemic AT-MSC administration on A375 xenotransplants. However, no support was observed for human glioblastoma cells 8MGBA co-injected along with AT-MSC that did not sustain tumor xenotransplant growth in vivo. Tumor-inhibiting response could be attributed to the synergistic action of multiple cytokines produced by AT-MSC on glioblastoma cells.Herein we provide experimental evidence for MSC-mediated protective effect on melanoma A375 cells under nutrient-limiting and hostile environmental conditions resulting from mutual crosstalk between neoplastic and non-malignant cells. This tumor-favouring effect was not observed for the glioblastoma cells 8MGBA. Collectively, our data further strengthen the need for unravelling mechanisms underlying MSC-mediated modulation of tumor behaviour for possible future MSC clinical use in the context of malignant disease.Mesenchymal stromal cells (MSC) represent a heterogeneous population of multi-potent cells with beneficial properties for regenerative processes and/or immunomodulation [1]. Therapeutic benefit for patients suffering from a wide range of severe pathologic conditions was reported in clinical trials employing MSC and derivatives thereof
%U http://www.molecular-cancer.com/content/9/1/129