%0 Journal Article
%T Rapid and efficient genetic manipulation of gyrencephalic carnivores using in utero electroporation
%A Hiroshi Kawasaki
%A Lena Iwai
%A Kaori Tanno
%J Molecular Brain
%D 2012
%I BioMed Central
%R 10.1186/1756-6606-5-24
%X Here we developed and validated a rapid and efficient technique that enables genetic manipulations in the brain of gyrencephalic carnivores using in utero electroporation. Transgene-expressing ferret babies were obtained within a few weeks after electroporation. GFP expression was detectable in the embryo and was observed at least 2£¿months after birth. Our technique was useful for expressing transgenes in both superficial and deep cortical neurons, and for examining the dendritic morphologies and axonal trajectories of GFP-expressing neurons in ferrets. Furthermore, multiple genes were efficiently co-expressed in the same neurons.Our method promises to be a powerful tool for investigating the fundamental mechanisms underlying the development, function and pathophysiology of brain structures which are unique to higher mammals.
%K Ferrets
%K Cerebral cortex
%K in utero electroporation
%U http://www.molecularbrain.com/content/5/1/24/abstract