%0 Journal Article
%T TGF-¦Â regulates invasive behavior of human pancreatic cancer cells by controlling Smad expression
%A Hirozumi Sawai
%A Akira Yasuda
%A Nobuo Ochi
%A Hiroki Takahashi
%J Archives of Medical Science
%D 2007
%I Termedia Publishing House
%X Introduction: To investigate the role of Smads in tumor cell activation, we examined changes in Smad expression as well as changes in proliferative and invasive behaviors in transforming growth factor-¦Â (TGF-¦Â) ¨C stimulated pancreatic cancer cells. Material and methods: Expression of TGF-¦Â receptor type I (T¦ÂR-I) and type II (T¦ÂR-II) was determined using RT-PCR and Western blot analysis in the human pancreatic cancer cell lines BxPC-3, Capan-2, and PANC-1. TGF-¦Â-mediated changes in Smad mRNA expression were examined using quantitative real-time RT-PCR. Proliferation of pancreatic cancer cells was monitored using an MTT assay and cell counting. Invasive behavior was examined using a Matrigel double-chamber assay. Results: T¦ÂR-I and T¦ÂR-II were expressed in all three cell lines studied here at the mRNA and protein level. Smad2/3 mRNA expression was decreased after TGF-¦Â stimulation in all three cell lines, while Smad4 mRNA expression remained unchanged. Smad6/7 mRNA expression was also attenuated in all three cell lines. TGF-¦Â enhanced the invasive capacity of all three cell lines, but had no effect on the proliferative behavior. Anti-T¦ÂR-II antibody inhibited this TGF-¦Â-enhanced invasive potential in pancreatic cancer cells. Conclusions: The Smad pathway, particularly down-regulation of Smad2/3 and Smad6/7, may be responsible for TGF-¦Â-induced invasion of human pancreatic cancer cells.
%K tumor invasion
%K pancreatic cancer
%K growth factor
%U http://www.termedia.pl/magazine.php?magazine_id=19&article_id=9000&magazine_subpage=FULL_TEXT