%0 Journal Article
%T Immunophoretic rapid diagnostic tests as a source of immunoglobulins for estimating malaria sero-prevalence and transmission intensity
%A Geoffrey S Williams
%A Clement Mweya
%A Laveta Stewart
%A George Mtove
%A Hugh Reyburn
%A Jackie Cook
%A Patrick H Corran
%A Eleanor M Riley
%A Chris J Drakeley
%J Malaria Journal
%D 2009
%I BioMed Central
%R 10.1186/1475-2875-8-168
%X Immunoglobulins eluted from RDTs, designed to detect parasite histidine rich protein-2 (HRP-2), were analysed by indirect ELISA for IgG recognizing the P. falciparum blood stage antigens merozoite surface protein-119 (MSP-119) and apical membrane antigen-1 (AMA-1). Optimal storage conditions for RDTs were evaluated by comparing antibody responses from RDTs stored in dry or humid conditions at 4ˇăC or at ambient temperature (with or without air-conditioning) for 7, 31 or 70 days. Antibody levels estimated using 3,700 RDT samples from attendees at health facilities in North-eastern Tanzania were compared with contemporaneously collected filter paper blood spots (FPBS) and used to estimate seroconversion rates.Storage of RDTs at 4ˇăC was optimal for immunoglobulin recovery but short-term storage at ambient temperatures did not substantially affect anti-malarial IgG levels. Results from RDTs were comparable with those from FPBSs, for both antigens. RDT-generated titres tended to be slightly higher than those generated from FPBSs, possibly due to greater recovery of immunoglobulins from RDTs compared to filter paper. Importantly, however, RDT-based seroconversion rates, and hence serological estimates of malaria transmission intensity, agreed closely with those from FPBSs.RDTs represent a practical option for collecting blood for sero-epidemiological surveys, with potential cost and logistical advantages over filter paper and other blood collection methods. RDT-based seroepidemiology can be incorporated into routine monitoring of malaria endemicity, providing information to supplement parasite prevalence rates and generating rapid, robust assessment of malaria transmission intensity at minimal extra cost.Accurate assessment of malaria transmission intensity is important in order to focus, monitor and assess the effectiveness of malaria control initiatives. The 'gold standard' measure of malaria transmission intensity is the entomological inoculation rate (EIR), i.e. the nu
%U http://www.malariajournal.com/content/8/1/168