%0 Journal Article
%T Dietary red palm oil supplementation reduces myocardial infarct size in an isolated perfused rat heart model
%A Dirk J Bester
%A Krisztina Kupai
%A Tamas Csont
%A Gergu Szucs
%A Csaba Csonka
%A Adriaan J Esterhuyse
%A Peter Ferdinandy
%A Jacques Van Rooyen
%J Lipids in Health and Disease
%D 2010
%I BioMed Central
%R 10.1186/1476-511x-9-64
%X Male Wistar rats were divided into three groups and fed a standard rat chow diet (SRC), a SRC supplemented with RPO, or a SRC supplemented with sunflower oil (SFO), for a five week period, respectively. After the feeding period, hearts were excised and perfused on a Langendorff perfusion apparatus. Hearts were subjected to thirty minutes of normothermic global ischaemia and two hours of reperfusion. Infarct size was determined by triphenyltetrazolium chloride staining. Coronary effluent was collected for the first ten minutes of reperfusion in order to measure MMP2 activity by gelatin zymography.Dietary RPO-supplementation decreased myocardial infarct size significantly when compared to the SRC-group and the SFO-supplemented group (9.1 ¡À 1.0% versus 30.2 ¡À 3.9% and 27.1 ¡À 2.4% respectively). Both dietary RPO- and SFO-supplementation were able to decrease MMP2 activity when compared to the SRC fed group. PKB/Akt phosphorylation (Thr 308) was found to be significantly higher in the dietary RPO supplemented group when compared to the SFO supplemented group at 10 minutes into reperfusion. There was, however, no significant changes observed in ERK phosphorylation.Dietary RPO-supplementation was found to be more effective than SFO-supplementation in reducing myocardial infarct size after ischaemia/reperfusion injury. Both dietary RPO and SFO were able to reduce MMP2 activity, which suggests that MMP2 activity does not play a major role in protection offered by RPO. PKB/Akt phosphorylation may, however, be involved in RPO mediated protection.Little is known about the effects of dietary edible oil supplementation on myocardial infarct size. Previous studies demonstrated that dietary RPO supplementation offers protection against ischaemia/reperfusion injury by improved aortic output recovery [1-4]. Engelbrecht and co-workers (2006) found that 6 weeks of dietary RPO supplementation was associated with increased PKB/Akt and p38 phosphorylation and decreased phosphorylation of
%U http://www.lipidworld.com/content/9/1/64