%0 Journal Article
%T Visceral fat mass determination in rodent: validation of dual-energy x-ray absorptiometry and anthropometric techniques in fat and lean rats
%A Maude Gerbaix
%A Lore Metz
%A Emeline Ringot
%A Daniel Courteix
%J Lipids in Health and Disease
%D 2010
%I BioMed Central
%R 10.1186/1476-511x-9-140
%X Forty-three Wistar male rats from different body weight, fat mass and ages were included in the study. Visceral fat mass was assessed by weighing the total perirenal and peri-epididymal adipose tissues after dissection. Statistical methods were used to discriminate the best region of interest allowing the in-vivo measure of Central Fat Mass by DXA. Abdominal circumference was measured at the same time as the DXA scan.A region of interest including Central Fat Mass from the whole body DXA scan (extending from L2 to L5 vertebrae), correlated strongly with ex-vivo Fat Mass (r = 0.94, p < 0.001). Abdominal circumference correlated significantly with ex-vivo Fat Mass (r = 0.82, p < 0.001) and Central Fat Mass (0.90, p < 0.001) in the whole group of rats. When dividing the whole group into lean and fat rats, correlations remained significant between Central Fat Mass and ex-vivo Fat Mass but disappeared for the lean group between abdominal circumference and ex-vivo Fat Mass.This study validates the Central Fat Mass determined by DXA as a non-sacrificial technique to assess visceral fat for in-vivo investigations in rats. The abdominal circumference measure appears useful in studying overweight or obese rats. These two techniques could be convenient tools in follow-up and longitudinal studies.Ageing, characterized by an increase of lipid consumption and sedentary lifestyle is known to be linked with metabolic pathologies. The excess of lipid provided by food is stored in localized sites (abdomen, liver, hips) and becomes pathological, disturbing a great number of metabolic functions and contributing to the rise in metabolic syndrome [1]. Abdominal obesity is also strongly linked with diabetes mellitus and an increase of cardiovascular disease, resulting in a prevalence of adverse cardiovascular events [2,3].The mechanisms leading to the local accumulation of adipose tissue are not fully elucidated in both humans and animals. Therefore, the main issue for clinicians or resea
%U http://www.lipidworld.com/content/9/1/140