%0 Journal Article
%T A rapid method for determining arachidonic:eicosapentaenoic acid ratios in whole blood lipids: correlation with erythrocyte membrane ratios and validation in a large Italian population of various ages and pathologies
%A Angela M Rizzo
%A Gigliola Montorfano
%A Manuela Negroni
%A Laura Adorni
%A Patrizia Berselli
%A Paola Corsetto
%A Klaus Wahle
%A Bruno Berra
%J Lipids in Health and Disease
%D 2010
%I BioMed Central
%R 10.1186/1476-511x-9-7
%X The ratio of total n-6:n-3 LCPUFA and arachidonic acid:eicosapentaenoic acid (AA and EPA), should not exceed 5:1. Increased intake of n-6 and decreased consumption of n-3 has resulted in much higher, ca 10/15:1 ratio in RBC fatty acids with the possible appearance of a pathological "scenario". The determination of RBC phospholipid LCPUFA contents and ratios is the method of choice for assessing fatty acid status but it is labour intensive and time consuming.[i] To describe and validate a rapid method, suitable for large scale population studies, for total blood fatty acid assay; [ii] to verify a possible correlation between total n-6:n-3 ratio and AA:EPA ratios in RBC phospholipids and in whole-blood total lipids, [iii] to assess usefulness of these ratio as biomarkers of LCPUFA status.[1] Healthy volunteers and patients with various pathologies were recruited.[2] Fatty acid analyses by GC of methyl esters from directly derivatized whole blood total lipids and from RBC phospholipids were performed on fasting blood samples from 1432 subjects categorised according to their age, sex and any existing pathologies.AA:EPA ratio and the total n-6:n-3 ratio were determined.AA:EPA ratio is a more sensitive and reliable index for determining changes in total blood fatty acid and it is correlated with the ratio derived from extracted RBC phospholipids.The described AA:EPA ratio is a simple, rapid and reliable method for determining n-3 fatty acid status.The evidence that diet is a key component of general health is well accepted. Governments are developing health strategies that propose to modify the diet and lifestyle of their citizens in order to reduce the incidence of diet-related conditions such as obesity, cardiovascular disease, cancer, type 2 diabetes and mental health problems and reduce the health-associated social and healthcare costs.Food manufacturers have reacted to the new market demands for healthier products in two ways. Firstly, they have attempted to eliminat
%U http://www.lipidworld.com/content/9/1/7