%0 Journal Article
%T Effect of HDL composition and particle size on the resistance of HDL to the oxidation
%A Nakanishi Shuhei
%A Sanni S£¿derlund
%A Matti Jauhiainen
%A Marja-Riitta Taskinen
%J Lipids in Health and Disease
%D 2010
%I BioMed Central
%R 10.1186/1476-511x-9-104
%X We studied HDL composition, subclass distribution, and the kinetics of CuSO4-induced oxidation of total HDL and HDL3 in vitro in 36 low-HDL-C subjects and in 41 control subjects with normal HDL-C.The resistance of HDL3 to oxidation, as assessed from the propagation rate was significantly higher than that of total HDL. The propagation rate and diene formation during HDL oxidation in vitro was attenuated in HDL derived from low-HDL-C subjects. Propagation rate and maximal diene formation during total HDL oxidation correlated significantly with HDL mean particle size. The propagation rate of total HDL oxidation in vitro displayed a significant positive association with HDL2 particle mass and HDL mean particle size by multiple regression analyses.These observations highlight that the distribution of HDL subpopulations has important implications for the potential of HDL as an anti-oxidant source.A strong body of epidemiological evidence associates low levels of high density lipoprotein cholesterol (HDL-C) with the cardiovascular disease risk [1,2]. Importantly, it was just recently reported that HDL protects against cardiovascular disease in both males and females, independent on subject's age and at all levels of risk [3].HDL protects against atherosclerosis by several mechanisms although the reverse cholesterol transport plays the major role. HDL exerts anti-atherogenic actions also via its inherent anti-oxidative and anti-inflammatory properties [4], because it carries enzymes such as paraoxonase 1 (PON1), which may protect against oxidation as well as inflammation [4-6]. Recently it was also demonstrated that apoA-I and HDL can neutralize procoagulant properties of anionic phospholipids and thereby prevent inappropriate stimulation of blood coagulation [7]. HDL acts as an effective scavenger of superoxide anions [8], and thus it can protect low density lipoprotein (LDL) against oxidative modification in vitro [5,6,9,10]. The interplay between LDL oxidation and athero
%U http://www.lipidworld.com/content/9/1/104