%0 Journal Article
%T The effects of ethanol and vasopressin on renal function in the isolated perfused rat kidney
%A M. Heringlake
%A J. Sehested
%A L. Bahlmann
%A H. Paarmann
%J Applied Cardiopulmonary Pathophysiology
%D 2011
%I Pabst Science Publishers
%X Controversial results regarding the role of vasopressin as a mediator of ethanol-induced diuresis raise the question, whether ethanol may stimulate diuresis directly on the level of the kidney, possibly by its vasodilating properties. We thus studied the effects of different ethanol-concentrations (0.5 ㏑, 1 ㏑, 2 ㏑, and 4 ㏑; n = 4 to 6, respectively) 每 in the presence of 10 mU/l vasopressin in the perfusion medium 每 and the effect of absence of vasopressin (-VP; n = 5) in isolated rat kidneys perfused for 180 minutes in a closed circuit system. A control group (con+VP; n = 6) was studied with the standard perfusion medium containing 10 mU/l vasopressin. Urine flow (UV) and free water clearance (CH2O) were increased (UV: con+VP: 124 ㊣ 78 vs. 每VP: 258 ㊣ 88 ml*min-1*g kidney-1; CH2O: - con+VP: 20.4 ㊣ 7.4 vs. 每VP: 51.6 ㊣ 20.8 ml*min-1*g kidney-1, p < 0.05 respectively) and fractional reabsorption of sodium (FRNa) were increased in rats perfused without vasopressin (FRNa: con+VP: 83.7 ㊣ 8.3 vs. 每VP: 75.0 ㊣ 2.6 %, p < 0.05). Ethanol-treatment had no effect on urine flow, sodium and potassium excretion, glomerular filtration rate, and renal vascular resistance. These data show that ethanol-mediated diuresis cannot be explained by intrinsic effects of ethanol on the kidney, at least in an isolated and denervated perfused rat kidney model.
%K ethanol-induced diuresis
%K fluid homeostasis
%K renal function
%U http://www.applied-cardiopulmonary-pathophysiology.com/fileadmin/downloads/acp-2011-1_20110329/03_heringlake.pdf