%0 Journal Article
%T In vivo cell aggregations of a recent swine biofilm-forming isolate of Leptospira interrogans strain from Argentina Agregaciones celulares in vivo de Leptospira interrogans producidas por un aislamiento porcino capaz de formar biofilm
%A Bibiana Brihuega
%A Luis Samartino
%A Carmelo Auteri
%A Agust赤n Venzano
%J Revista argentina de microbiologˋ-a
%D 2012
%I Asociaci車n Argentina de Microbiolog赤a
%X Leptospirosis is a zoonosis of ubiquitous distribution caused by spirochetes. Leptospires exist either as saprophytic water-associated organisms or as animal pathogens that can survive in water. Previous works have demonstrated that both saprophytic and pathogenic leptospires are able to produce functional biofilms, which consist of a community of bacteria embedded in an extracellular matrix attached to a surface. This structure is believed to provide protection from environmental aggressiveness. In the present study, we analyzed the capacity of biofilm formation both of a a recent field isolate of Leptospira interrogans serovar Pomona obtained from an aborted swine fetus and of the saprophytic Leptospira biflexa serovar Patoc. We used light microscopy, immunofluorescence, and scanning electron microscopic examinations on glass and polystyrene plate models to evaluate the process in vitro. The ability to form bacterial aggregations in vivo was tested using pregnant guinea pigs infected with both strains. We obtained biofilms both on glass and plastic surfaces. Scanning electron microscopic analysis showed differences in the biofilm structure formed by both strains. L. interrogans serovar Pomona cell aggregations were observed in placental tissues by light microscopy. Biofilms and cell aggregations are consistent with the life of saprophytic strains in water and could help pathogenic strains to colonize the host and lead to abortion in pregnant animals. La leptospirosis es una zoonosis de amplia distribuci車n causada por el g谷nero Leptospira. Las leptospiras existen de manera sapr車fita asociadas a ambientes acu芍ticos o como pat車genos animales que tambi谷n pueden sobrevivir en el agua. Trabajos previos demostraron que tanto las leptospiras sapr車fitas como las pat車genas tienen la capacidad de formar biofilms, que consisten en una comunidad de bacterias embebidas en una matriz extracelular adherida a una superficie. Esta estructura tendr赤a la funci車n de proveer protecci車n contra el medioambiente. En este estudio, analizamos la capacidad de formar biofilm en un aislamiento obtenido recientemente de un feto porcino abortado, caracterizado como Leptospira interrogans serovar Pomona, y en la bacteria sapr車fita Leptospira biflexa serovar Patoc. Se estudi車 la formaci車n de biofilm en distintas superficies (vidrio y poliestireno), las que se evaluaron por microscop赤a 車ptica, inmunofluorescencia y microscop赤a electr車nica de barrido. La capacidad de formar agregaciones bacterianas in vivo se evalu車 utilizando un modelo de cobayas pre adas infectadas con ambas cepas. Se
%K Leptospira interrogans
%K Biofilms
%K Agregaciones celulares
%K Cobayo
%K Leptospira interrogans
%K Biofilms
%K Cell aggregations
%K Guinea pigs
%U http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412012000300002