%0 Journal Article
%T Evaluaci車n del antibiograma directo desde el frasco de hemocultivo con el sistema Vitek 2C: su utilidad en la cl赤nica Evaluation of direct susceptibility testing from blood culture bottles: Clinical usefulness
%A Rolando N. Soloaga
%A Natalia A. Carri車n
%A Alejandra M. Margari
%A Marta Giovanakis
%J Revista argentina de microbiologˋ-a
%D 2012
%I Asociaci車n Argentina de Microbiolog赤a
%X Se realiz車 un estudio prospectivo observacional que incluy車 191 episodios de bacteriemia monomicrobiana por bacilos gram negativos en los que se combin車 el uso del sistema Bact-Alert de hemocultivos con el sistema de identificaci車n y sensibilidad Vitek 2C. Se compararon los resultados con los correspondientes a los de colonia aislada en medios s車lidos. La correlaci車n obtenida en la identificaci車n fue del 99 %. Con respecto a la sensibilidad a los antibi車ticos, la correlaci車n total en categor赤a fue del 99 % (0,22 % de errores muy mayores, 0,17 % de errores mayores y 0,61 % de errores menores). De los 191 episodios de infecciones del torrente sangu赤neo, 108 (56,5 %) recibieron los antibi車ticos adecuados en forma emp赤rica. Una vez comunicado el resultado del antibiograma directo del frasco, se cambi車 el tratamiento antibi車tico en 116 episodios (60,7 %). A prospective observational study was conducted in two hospitals of Buenos Aires city (Argentina); 191 clinically significant monomicrobial gram-negative bloodstream infections were included in the study, which combined the Bact-Alert System Blood culture machine and the Vitek 2C System. Organism identification and susceptibility results directly from blood culture bottles were compared with those obtained from cards inoculated with a standardized bacterial suspension obtained following subculture on agar. By comparing the results obtained from pure cultures with those by the Vitek 2C System as reference method, the agreement between the reference method and the direct identification from positive blood cultures was 99 %. By antimicrobial susceptibility testing, the overall categorical accuracy was 99 % (0.22 %, very major errors, 0.17 %, major errors and 0.61 %, minor errors). One hundred and eight (56,8 %) bloodstream infections were treated empirically with adequate antibiotics. After the results obtained directly from the bottles were reported, antimicrobial therapy was changed in 116 (60.7 %) of the episodes.
%K Bacilos gram negativos
%K Bacteriemia
%K Vitek 2C
%K Gram-negative rods
%K Bacteremia
%K Vitek 2C
%U http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412012000300006