%0 Journal Article
%T Heterogeneity of 3¡¯-Untraslated Region of Genome RNA of the Tick-Borne Encephalitis Virus (TBEV) Strains Isolated from Ticks in the Western Siberia, Russia
%A Olga V. Morozova
%A Valentina N. Bakhvalova
%A Igor V. Morozov
%J International Journal of Biomedical Science
%D 2007
%I 
%X The tick-borne encephalitis virus (TBEV) strains have been isolated from unfed adult ticks Ixodes persulcatus Schulze in Novosibirsk region (South-Western Siberia, Russia) beginning from 1980 till 2006. The TBEV 3¡¯-untraslated region (3¡¯UTR) variable fragment was amplified with primers corresponding to conservative flanking areas. The RT-PCR product lengths varied in range from 100 to 400 bp. Comparative analysis of 3¡¯UTR nucleotide sequences revealed a few groups of the TBEV strains within Siberian genetic subtype with significant intra-group homology and essential differences between groups. Correlation between lengths of the 3¡¯UTR fragments and hemagglutination (HA) titers for subsequent passages of the TBEV strains was not found. However, for the viral strains with shorter 3¡¯UTR (less than 200 nucleotides) incubation period for suckling mice was longer than 5 days. It might be resulted from decreased RNA synthesis or reduced neuroinvasiveness.
%K deletions
%K hemagglutination
%K neurovirulence
%K reverse transcription¨Cpolymerase chain reaction
%K tick-borne encephalitis virus strains
%K 3¡¯untraslated region sequences
%U http://ijbs.org/User/ContentFullTextFrame.aspx?VolumeNo=3&StartPage=206