%0 Journal Article
%T Mutagenesis of a Copper P-Type ATPase Encoding Genein Methylococcus capsulatus (Bath) Results inCopper-Resistance
%A Ashraf Y. Z. Khalifa
%J International Journal of Bioscience, Biochemistry and Bioinformatics
%D 2013
%I IACSIT Press
%R 10.7763/ijbbb.2013.v3.159
%X Copper is an essential micronutrient for all living cells, however, it is extremely toxic at high concentrations and thus copper homeostasis is required to be tightly regulated. copA encodes for a copper-translocating P-type ATPase (CopA) and plays a vital role in copper homeostasis and is involved in copper transport across membranes of many organisms. Little is known about copper homeostasis in Methylococcus capsulatus although copper has a significant physiological role in this methanotroph. In this study we investigated the disruption of a CopA1 homologue (MCA2072; copA1) in M. capsulatus (Bath) by insertional inactivation mutagenesis. The resulting mutant, M. capsulatus ¦¤copA1, was copper resistant to elevated copper concentrations (100 ¦ÌM) than the wild-type strain (80 ¦ÌM). Furthermore, the intracellular copper measurements revealed that ¦¤copA1 accumulated half the amount of copper when compared with the wild-type. No observed phenotypic difference between the mutant strain and wild-type related to growth at different silver concentrations. These observations suggest that M. capsulatus CopA1 has a key role in copper homeostasis.
%K CopA
%K copper homeostasis
%K P-type ATPases
%K Methylococcus capsulatus
%U http://www.ijbbb.org/papers/159-CB418.pdf