%0 Journal Article
%T Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) ¡®Kluai Sa¡¯ and ¡®Kluai Leb Mu Nang¡¯
%A Kamnoon KANCHANAPOOM
%A Korn KORAPATCHAIKUL
%J Notulae Scientia Biologicae
%D 2012
%I University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca
%X Yellow compact calluses were induced from in vitro-grown shoot tips of diploid bananas (Musa acuminata, AA group) ¡®Kluai Sa¡¯ and ¡®Kluai Leb Mu Nang¡¯ on a modified Murashige and Skoog (MS) medium containing 100 mg/L malt extract, 50 mg/L proline, 50 mg/L cysteine, 100 mg/L glutamine, 1 mg/L biotin, 7 mg/L Dicamba and 2 mg/L TDZ. Green shoot buds were induced after transfer of the yellow compact calluses to the same MS medium but supplemented with 1 mg/L NAA and 3 mg/L BA and plant regeneration was achieved through organogenesis in callus cultures. Regenerated shoots were rooted on MS medium containing 0.2% activated charcoal but without plant growth regulators. Histological analysis revealed that calluses originated from small dense cells with well stained cytoplasm and nucleus typical of meristematic cells.
%U http://notulaebiologicae.ro/index.php/nsb/article/view/7223