%0 Journal Article
%T Optimization of solvent extraction conditions for total carotenoids in rapeseed using response surface methodology
%A Ling Wang
%A Yun Liu
%J Natural Science
%P 23-29
%@ 2150-4105
%D 2009
%I Scientific Research Publishing
%R 10.4236/ns.2009.11005
%X The optimum total carotenoids (TC) extraction from rapeseed with solvent extraction method by UV-visible spectrophotometer determination was investigated by using response surface methodology (RSM). Extraction duration, re-peated extraction cycles, solvent-solid ratio and extraction temperature were assumed to be the most important factors affecting solvent extrac-tion for the determination of TC. Optimum sol-vent extraction conditions for maximizing the determination of TC were: extraction duration 7.3h, repeated extraction three times, ratio of solvent-solid (v/w, mL/mg) 29:1, extraction temperature 42°C. Under the optimal conditions, the yield of TC was up to 4.79 mg /100g. The model had a satisfactory coefficient of R2 (= 0.912) and verified experimentally. The results showed that the conditions were mild and use-ful for maximizing a quantitative spectropho-tometer determination of TC in rapeseed.
%K Carotenoids
%K Optimization
%K Solvent Extraction
%K Response Surface Methodology
%K Rapeseed
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=500