%0 Journal Article
%T Production of pectinase by Aspergillus niger cultured in solid state media.
%A Nazneen Akhter 1
%A M. Alam Morshed 1
%A 3*
%A Azim Uddin 3
%A Feroza Begum 2
%A Tipu Sultan 1
%A Abul Kalam Azad 1
%J International Journal of Biosciences
%D 2011
%I International Network for Natural Sciences
%X Solid state fermentation was carried out with 7 fungal strains, obtained from different sources. Among 7 isolates Aspergillus niger, IM-6 was found as effective pectinase producer. Maximum enzymatic activity (142.44U/gm) was observed after 7 days incubation at 40 C temperature in 750 ml conical flask. In this study 1.69% (NH4)2SO4 was used as nitrogen source, although peptone as a nitrogen source showed better result but use of peptone was not cost effective. As a substrate, wheat bran and potato starch showed good result (85.54U/gm) in solid state culture. Addition of 9.68% pectin was found to increase the enzyme production as 116.57U/gm. Pectinase production was optimum in 60% moisture (98.34U/gm). Aeration showed positive effects on pectinase production (136.86U/gm) at 750 ml flask than 1000 ml flask. Thus the wild strain Aspergillus niger IM-6 has outstanding pectinase producing capability at 40 C in 60% initial moisture content for 7 days of incubation insolid state fermentation.
%K Pectinase
%K Pectin
%K Aspergillus sp. Solid state fermentation.
%U http://innspub.net/documents/IJB-V1No1-p33-42.pdf