%0 Journal Article
%T IL-6 Induction by TNF¦Á and IL-1¦Â in an Osteoblast-Like Cell Line
%A Elena Confalone
%A Adriana Furia
%A Giuseppe D¡äAlessio
%J International Journal of Biomedical Science
%D 2011
%I 
%X Stress cytokines tumor necrosis factor ¦Á, interleukin-1¦Â and interleukin-6 modulate the activity of a variety of cell types including osteoblasts, and are involved in the pathogenesis of several rheumatic diseases associated with systemic bone loss. We have studied the expression of interleukin-6 induced by interleukin-1¦Â and tumor necrosis factor ¦Á in the osteoblast-like cell line MG-63, derived from a human osteosarcoma. We have observed marked differences in the regulation of interleukin-6 gene expression by tumor necrosis factor ¦Á or interleukin-1¦Â. at the level of mRNA steady state and stability and cytokine secretion. In addition, N-acetyl cysteine, a scavenger of reactive oxygen species, inhibits activation of NF-¦ÊB and induction of interleukin-6 by tumor necrosis factor ¦Á, being ineffective on interleukin-1 ¦Â activity. These data illustrate the action of stress cytokines on a cell line widely used in in vitro studies as a reliable model of osteoblast response to cytokines involved in bone resorbing diseases, an important issue for developing new strategies for treatments of bone diseases
%K stress cytokines
%K antioxidant
%K osteosarcoma cells
%U http://www.ijbs.org/User/ContentFullTextFrame.aspx?VolumeNO=6&StartPage=135