%0 Journal Article
%T Targeting of ICAM-1 on vascular endothelium under static and shear stress conditions using a liposomal Gd-based MRI contrast agent
%A Leonie E Paulis
%A Igor Jacobs
%A Nynke van de Akker
%A Tessa Geelen
%A Daniel Molin
%A Lucas W Starmans
%A Klaas Nicolay
%A Gustav J Strijkers
%J Journal of Nanobiotechnology
%D 2012
%I BioMed Central
%R 10.1186/1477-3155-10-25
%X A paramagnetic liposomal contrast agent functionalized with anti-ICAM-1 antibodies for multimodal magnetic resonance imaging (MRI) and fluorescence imaging of endothelial ICAM-1 expression is presented. The ICAM-1-targeted liposomes were extensively characterized in terms of size, morphology, relaxivity and the ability for binding to ICAM-1-expressing endothelial cells in vitro. ICAM-1-targeted liposomes exhibited strong binding to endothelial cells that depended on both the ICAM-1 expression level and the concentration of liposomes. The liposomes had a high longitudinal and transversal relaxivity, which enabled differentiation between basal and upregulated levels of ICAM-1 expression by MRI. The liposome affinity for ICAM-1 was preserved in the competing presence of leukocytes and under physiological flow conditions.This liposomal contrast agent displays great potential for in vivo MRI of inflammation-related ICAM-1 expression.
%K Molecular MRI
%K Liposome
%K ICAM-1
%K Endothelium
%K Leukocyte
%K Shear stress
%U http://www.jnanobiotechnology.com/content/10/1/25/abstract