%0 Journal Article
%T A SIMPLE EXTRACTIONLESS REVERSE PHASE HPLC METHOD FOR DETECTION AND QUANTIFICATION OF CYPERMETHRIN IN RAT PLASMA
%A Ajay Kumar
%A Anu Rahal
%A Rajesh Mandil
%A Atul Prakash
%J International Journal of Bioassays
%D 2013
%I 
%X This study reports a simple and rapid High Performance Liquid Chromatography (HPLC) method for estimation of the common domestic insecticide, cypermethrin in rat plasma. The method is based upon a simple step extraction of pyrethroid from plasma and assay using reverse phase C18 column as solid phase, mixture of methanol and water (87:13) as mobile phase with a flow rate of 0.9ml/min and photodiode array detection at 225nm. The limit of detection and limit of quantitation were estimated to be 0.06 and 0.1¦Ìg.ml-1, respectively. The standard curve was linear in the concentration range of 0.1-15¦Ìg.ml-1 with a correlation of 0.997. This method is advantageous for routine analysis of pyrethroids in plasma.
%K Cypermethrin
%K Extractionless
%K HPLC
%K Method
%K Plasma
%K Rat
%U http://ebioscholar.com/ojs/index.php/ijb/article/view/220