%0 Journal Article
%T CLONING£¬SEQUENCE ANALYSIS AND INDUCED EXPRESSION STUDIES OF A CHITINASE GENE M-CHITINASE FROM MULBERRY (MORUS L.)
%A Wei Tong
%A Dongqing Hu
%A Heng Wang
%A Longi Li
%A Yuhua Wang
%A Yinghua Zhang
%A Zhaoyue Liu
%A Rongjun Fang and Weiguo Zhao*
%J International Journal of Bioassays
%D 2013
%I 
%X A full-length cDNA sequence coding for Chitinase in mulberry, which we designated M-chitinase (GenBank accession number: HQ117891) was cloned based on mulberry expressed sequence tags (ESTs) isolated from the cDNA library. Its full open reading frame was obtained by RACE and RT-PCR. Sequence analysis showed that the M-chitinase gene is 1392 bp in length and contains a 60 bp 5¡¯-UTR (un translated region) and a 255 bp 3¡¯-UTR. Its open reading frame (ORF) is of 1077 bp long, encoding 358 amino acids with a predicted molecular weight of 38.52KDa and an isoelectric point of 4.466. Homology analysis revealed that M-chitinase gene in mulberry is highly conservative with other species including N. khasiana, Zea mays and Zea Diploperennis. Phylogenetic analysis based on M-chitinase gene with other 19 species revealed that mulberry shows closer relationship with Nicotiana gossei, Nicotiana tabacum, Capsicum annuum and Arabis blepharophylla. The results of semi quantitative RT-PCR analysis showed that the mRNA transcriptional level of M-chitinase in the young leaf was changed significantly under the conditions of signal transduction mechanism underlying the stress response in mulberry.
%K Chitinase
%K cDNA library
%K Mulberry
%U http://ebioscholar.com/ojs/index.php/ijb/article/view/282