%0 Journal Article
%T Molecular phylogeny of angiospermic plant families using RBCL gene sequences
%A B. Uma Reddy
%J International Journal of Bioinformatics Research
%D 2009
%I Bioinfo Publications
%X The present study was undertaken to assess the role of plastid-rbcL (ribulose-1, 5-bisphosphatecarboxylase large-subunit) gene sequences in addressing the evolutionary relationships within theangiosperms at inter and intra-familial levels using computational experiment. In order to elucidate therelationships, a set of 92 chloroplast rbcL sequences representing from 90 taxa of 12 genera and 10angiospermic plant families (dicot and monocots) were withdrawn from the GenBank database. The multiplesequence alignment was performed using Genebee-ClustalW service to findout the regions of conserved orindels among the sequences. The phylogenetic tree was inferred from these sequences by employingBootstrap method of UPGMA (Unweighted Pair Group Method with Arithmetic Mean) using MEGA(Molecular Evolutionary Genetics Analysis) software. The consistency of these generic-wise groupings wasfurther confirmed by the MP (Maximum-Parsimony), ME (Minimum-Evolution) and NJ (Neighbor-Joining)methods. The analysis of these studies strongly indicates that, out of the 12 selected genera Trichosanthes(Cucurbitaceae), Phyllanthus (Phyllanthaceae), Austrobryonia (Cucurbitaceae), Solanum (Solanaceae),Piper (Piperaceae) and Saxifraga (Saxifragaceae) are grouped into separate clusters and exhibitingmonophyletic conditions. Where as, Drypetes (Putranjivaceae), Asparagus (Asparagaceae), Cassia(Caesalpinioideae, sub-family), Canna (Cannaceae), Mentha (Lamiaceae), are paraphyletic and themembers of the Salvia (Lamiaceae) are distributed throughout these hiraeoid clades, confirming thepolyphyly of this large genus. Similar observations were noticed in all four methods. Thus, chloroplast rbcLgene sequences can unambiguously resolve the relationships, as well as provided a good indication ofmajor supra-generic groupings among the selected angiospermic plant families and also gave many cluesfor future studies.
%K rbcL gene
%K monophyletic
%K paraphyletic
%K polyphyletic
%K Phylogenetic relationship
%K MEGA
%U http://www.bioinfo.in/uploadfiles/12585390921_2_4_Bioinfo.pdf