%0 Journal Article
%T Polymerase Chain Reaction Detection of C. pseudotuberculosis in the Brain of Mice Following Oral Inoculation
%A Faez Firdaus Jesse
%A Lawan Adamu
%A Abdinasir Yusuf Osman
%A Ahmad Fauzan Bin Muhdi
%J International Journal of Animal and Veterinary Advances
%D 2013
%I Maxwell Science Publication
%X The aim of present study was to detect the presence of C. pseudotuberculosis in the brain of the mice following oral inoculation as a model using PCR. Caseous lymphadenitis is a chronic and subclinical disease of sheep and goats which has universal distributions, presenting enormous animal and flock prevalence. Total of 16 mice were used for this study, 8 mice were inoculated orally with 1.0 mL sterile phosphate buffered saline pH 7, while another 8 mice were inoculated with 1.0 mL of 109 colony forming unit of C. pseudotuberculosis. Seven different organs were collected during post mortem for the detection ofC. pseudotuberculosis The result indicated 3 positive samples in lymph nodes, 5 in the brain and 1 in the liver. The PCR used in the present study may successfully be applied for the detection and diagnosis of C. pseudotuberculosis in the brain of the mice following oral inoculation.
%K Brain
%K C. pseudotuberculosis
%K detection
%K mice
%K oral inoculation
%K PCR
%U http://maxwellsci.com/jp/abstract.php?jid=IJAVA&no=282&abs=05