%0 Journal Article
%T Intra-coronary administration of tacrolimus markedly attenuates infarct size and preserves heart function in porcine myocardial infarction
%A Sarah Chua
%A Steve Leu
%A Jiunn-Jye Sheu
%A Yu-Chun Lin
%A Li-Teh Chang
%A Ying-Hsien Kao
%A Chia-Hung Yen
%A Tzu-Hsien Tsai
%A Yung-Lung Chen
%A Hsueh-Wen Chang
%A Cheuk-Kwan Sun
%A Hon-Kan Yip
%J Journal of Inflammation
%D 2012
%I BioMed Central
%R 10.1186/1476-9255-9-21
%X Twelve male mini-pigs were randomized into AMI-saline (MI-only) group and AMI-tacrolimus (MI-Tac) group that received intra-coronary saline (3.0£¿mL) and tacrolimus (0.5£¿mg in 2.5£¿mL saline) injection, respectively, beyond site of ligation 30 minutes after LAD occlusion.Larger infarct area was noted in MI-only group (p£¿<£¿0.001). Inflammatory biomarkers at protein [oxidative stress, tumor necrotic factor-¦Á, nuclear factor-¦ÊB], gene (matrix metalloproteinase-9, plasminogen activator inhibitor-1), and cellular (CD40+, CD68+ inflammatory cells) levels were remarkably higher in MI-only animals (p£¿<£¿0.01). Conversely, anti-inflammatory biomarkers at gene level (Interleukin-10), gene and protein level (endothelial nitric oxide synthase), and anti-oxidant biomarkers at both gene and protein levels [heme oxygenase 1, NAD(P)H:quinone oxidoreductase] were lower in MI-only group (p£¿<£¿0.01). Number of apoptotic nuclei and apoptotic biomarkers expressions at gene and protein levels (Bax, caspase 3) were notably higher, whereas anti-apoptotic biomarkers at gene and protein levels (Bcl-2), LVEF, and fractional shortening were markedly lower in MI-only group (p£¿<£¿0.001).Intra-coronary administration of tacrolimus significantly attenuated infarct size and preserved LV function.
%U http://www.journal-inflammation.com/content/9/1/21/abstract