%0 Journal Article
%T The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells
%A Chunxiao Ji
%A Fenglian Ren
%A Heng Ma
%A Ming Xu
%J Journal of Experimental & Clinical Cancer Research
%D 2010
%I BioMed Central
%R 10.1186/1756-9966-29-50
%X After the human HepG2 cells had been treated with the DADS and inhibitors for 24 h, cell viability was determined by the MTT method, apoptosis was evaluated by flow cytometry (FCM) and the expressions of p38MAPK and caspase-3 were measured by western-blot.Our results indicated that DADS activities the p38MAPK and caspase-3, but the inhibitors, SB203580 and Z-DEVD-FMK (for p38MAPKand for caspase-3, respectively), both have the effect of inhibitory activity on P38MAPK and caspase-3. Furthermore, a combination treatment with both DADS and inhibitor (SB203580 or Z-DEVD-FMK) decreases the inhibitory and apoptotic activity of HepG2 cells increased compared with DADS-treated.Our data indicate that p38MAPK and caspase-3 are involved in the process of DADS-induced apoptosis in human HepG2 cells and interact with each other.The MAPK (mitogen-activated protein kinase) system is a cluster of serine/threonine protein kinases in the cells, and the activitied MAPKs participate in a variety of cellular responses including genetic transcription, inducing cell apoptosis, maintaining cell and regulating cell cycle, and so on [1-3]. The p38MAPK is the key member of the MAPK family and more commonly activated in response to cytokines, stress and cellular damage [4,5]. A large number of studies have shown that the activity of p38MAPK is necessary in the apoptosis process induced by various anti-cancer drugs. Caspase enzymes play a very important role when cells started apoptosis as the central effector of apoptosis. Caspase-3, is the ultimate enforcer of apoptotic death, which can cleavage many proteins of important structure and function directly[6].Diallyl disulfide (DADS) is one kind of oil-soluble sulfur organic compounds, it is a potential broad-spectrum anti-cancer drug. Studies have shown that DADS can inhibit human tumor cells grow including those of colon, lung, skin, breast, liver origins and prostate [7-10]. There are also lots of reports about the caspase-3 involvement during
%U http://www.jeccr.com/content/29/1/50