%0 Journal Article
%T A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas in vivo and in vitro
%A Wei Sun
%A Yue Z Fan
%A Wen Z Zhang
%A Chun Y Ge
%J Journal of Experimental & Clinical Cancer Research
%D 2011
%I BioMed Central
%R 10.1186/1756-9966-30-46
%X The invasive potential of human gallbladder carcinoma cell lines GBC-SD and SGC-996 were identified by Transwell membrane. The vasculogenic-like network structures and the signal intensities i.e. hemodynamic in gallbladder carcinomas stimulated via the three-dimensional matrix of GBC-SD or SGC-996 cells in vitro, the nude mouse xenografts of GBC-SD or SGC-996 cells in vivo were observed by immunohistochemistry (H&E staining and CD31-PAS double staining), electron microscopy and micro-MRA with HAS-Gd-DTPA, respectively.Highly aggressive GBC-SD or poorly aggressive SGC-996 cells preconditioned by highly aggressive GBC-SD cells could form patterned networks containing hollow matrix channels. 85.7% (6/7) of GBC-SD nude mouse xenografts existed the evidence of VM, 5.7% (17/300) channels contained red blood cells among these tumor cell-lined vasculatures. GBC-SD xenografts showed multiple high-intensity spots similar with the intensity observed at tumor marginal, a result consistent with pathological VM.VM existed in gallbladder carcinomas by both three-dimensional matrix of highly aggressive GBC-SD or poorly aggressive SGC-996 cells preconditioned by highly aggressive GBC-SD cells in vitro and GBC-SD nude mouse xenografts in vivo.The formation of a microcirculation (blood supply) occurs via the traditionally recognized mechanisms of vasculogenesis (the differentiation of precursor cells to endothelial cells that develop de novo vascular networks) and angiogenesis (the sprouting of new vessels from preexisting vasculature in response to external chemical stimulation). Tumors require a blood supply for growth and hematogenous metastasis, and much attention has been focused on the role of angiogenesis [1]. Recently, the concept of "vasculogenic mimicry (VM)" was introduced to describe the unique ability of highly aggressive tumor cells, but not to poorly aggressive cells, to express endothelium and epithelium-associated genes, mimic endothelial cells, and form vascular chan
%K Gallbladder neoplasm
%K vasculogenic mimicry
%K 3-dimensional matrix
%K Xenograft model
%K Histomorphology
%K Hemodynamic
%U http://www.jeccr.com/content/30/1/46