%0 Journal Article
%T Tissue microarray analysis of eIF4E and its downstream effector proteins in human breast cancer
%A Heather E Kleiner
%A Prasad Krishnan
%A Jesse Tubbs
%A Mark Smith
%A Carol Meschonat
%A Runhua Shi
%A Mary Lowery-Nordberg
%A Patrick Adegboyega
%A Marcia Unger
%A James Cardelli
%A Quyen Chu
%A J Michael Mathis
%A John Clifford
%A Arrigo De Benedetti
%A Benjamin DL Li
%J Journal of Experimental & Clinical Cancer Research
%D 2009
%I BioMed Central
%R 10.1186/1756-9966-28-5
%X Breast tumor TMAs were stained immunohistochemically and quantitated using the ARIOL imaging system. In the TMAs, eIF4E levels correlated strongly with c-Myc, cyclin D1, TLK1B, VEGF, and ODC. Western blot comparisons of eIF4E vs. TLK1B were consistent with the immunohistochemical results. Consistent with our previous western blot results, eIF4E did not correlate with node status, ER, PR, or HER-2/neu.We conclude that the TMA technique yields similar results as the western blot technique and can be more efficient and thorough in the evaluation of several products downstream of eIF4E.Breast cancer remains a major cause of death among women. The American Cancer Society's facts and figures shows that 182,460 new cases of breast cancer will be diagnosed in women in 2008 [1]. The number of deaths due to breast cancer in 2008 is projected to be 40,480. In addition, 1990 men are expected to get breast cancer and 450 to die of it in 2008. There are several risk factors for breast cancer occurrence such as genetic susceptibility, radiation, obesity, and alcohol use. Pathways activated in breast cancer include Eukaryotic Translation Initiation Factor 4E (eIF4E) pathway [2], Phosphatidylinositol-3-kinase(PI3K)-AKT pathway [3], Mitogen-Activated Protein Kinase (MAPK) pathway [4] and the Nuclear factor-kappaB (NFkB) pathway [5]. Our research has focused on the role of the eIF4E in human breast cancer.The eukaryotic translation initiation factor, eIF4E, is a 25-kD cytosolic cap-binding protein that recognizes and binds to the 7-methylguanosine cap in the 5'-untranslated regions (5'-UTR) of mRNAs during the initiation of protein translation (reviewed in [6,7]). eIF4E may be considered the rate-limiting component in translation initiation because it is found in much lower amounts than other translation factors and is activated via mitogenic stimuli (serum, phorbol esters, tumor necrosis factor a, and lipopolysaccharide [6]). Several complex 5'-UTR mRNAs involved in cell division, ce
%U http://www.jeccr.com/content/28/1/5