%0 Journal Article
%T Accessible morphohistochemical labs-on-a-chip based on different counting chambers' grids: microfluidic morphodynamical workstations
%A Gradov O.V.
%A Notchenko A.V.
%J §®§à§â§æ§à§Ý§à§Ô£¿§ñ
%D 2012
%I Dnipropetrovsk Medical Academy of Ministry of Health of Ukraine, Ukrainian scientific Society of Anatomists, Histologists, Embryologists
%X An accessible design of autonomous labs on the chip which do not require a special reader is developed. The proposed device uses hemocytometric counting chambers for determinati on of concentration of cells, isolated by a special device for cell sedimentation. A system of automated Rf-identification of chambers in the long-term storage is proposed which provides new morphometric data at various stages of cultivation or biomonitoring. A ne w diffraction method of calcu-lation and fingerprinting of cellular structures in varying environmental conditions is described. Experimental data on the pilot testing of Fuchs-Rosenthal, Buerker, Neubauer, Makler and Thoma chambers fo r the described technique is given. The applicability of these devices for coherent-d ensitometric indication either of concen tration dynamics in cell culture or suspen -sion or morphogenetic processes in them for laminar conditions is shown. A self-learning program for cellular pattern recog-nition and relational cytophotometry based on lab on the chip w ith an object-micrometric calib ration relative to the counting net is demonstrated. A hybrid in vitro / in silico met hod of morphogenesis monitoring in cell cultures is introduced.
%K lab-on-a-chip
%K haemocytometer
%K counting chambers
%K hematimeter
%K mathematical morphology
%K computer morphometry
%K 3D luminance surface
%K digital morphogenesis
%U http://www.morphology.dp.ua/_pub/MORPHO-2012-06-01/12govmrs.pdf