%0 Journal Article
%T Molecular Characterization of Wheat Genotypes Using SSR Markers
%A Sheikh Arslan Sehgal
%A Rana Adnan Tahir
%A Muhammad Nawaz
%J International Journal Bioautomation
%D 2012
%I Academic Publishing House
%X Wheat (Triticum aestivum L.) rusts are the most destructive and widespread among all other diseases of wheat because of their wide distribution, and their capacity to form new races that can attack previously resistant cultivars which result in serious yield losses. The molecular characterization and genetic diversity of 20 wheat genotypes was investigated using 34 polymorphic Simple Sequence Repeats (SSR) screened primers. About thirty-one loci were found. Lr-19 gene was present in all 20 wheat genotypes that cause resistance against wheat rust. Shalimar-86 and Chakwal-86 showed the highest genetic diversity with SH-02 and Ufaq respectively, giving a 98.94% genetic similarity and a minimum genetic diversity was observed between Chakwal-50 and Bhakar which showed that they are 74% similar. The current research found that SSR makers could distinguish and characterize all of the genotypes, more screened primers could be used for study and for saturation of different regions in further research. The identification of rust resistant genes in Pakistani wheat germplasm will help in accelerating the breeding program in future, including pyramiding of different wheat resistant genes in wheat genotypes and varieties.
%K Molecular characterization
%K Wheat rust
%K SSR markers
%U http://www.clbme.bas.bg/bioautomation/2012/vol_16.2/files/16.2_03.pdf