%0 Journal Article
%T Regeneration of Centella asiatica plants from non-embryogenic cell lines and evaluation of antibacterial and antifungal properties of regenerated calli and plants
%A Yamin Bibi
%A Muhammad Zia
%A Sobia Nisa
%A Darima Habib
%A Abdul Waheed
%A Fayyaz M Chaudhary
%J Journal of Biological Engineering
%D 2011
%I BioMed Central
%R 10.1186/1754-1611-5-13
%X Present study reveals that 6-benzyladenine (BA) either alone or in combination with 1-naphthalene acetic acid (NAA) supplemented in Murashige and Skoog (MS) medium at different concentrations produced good quality callus from leaf explants of C. asiatica. The calli produced on different plant growth regulators at different concentrations were mostly embryogenic and green. Highest shoot regeneration efficiency; 10 shoots per callus explant, from non-embryogenic callus was observed on 4.42 ¦ÌM BA with 5.37 ¦ÌM NAA. Best rooting response was observed at 5.37 and 10.74 ¦ÌM NAA with 20 average number of roots per explant. Calli and regenerated plants extracts inhibited bacterial growth with mean zone of inhibition 9-13 mm diameter when tested against six bacterial strains using agar well diffusion method. Agar tube dilution method for antifungal assay showed 3.2-76% growth inhibition of Mucor species, Aspergillus fumigatus and Fusarium moliniformes.The present investigation reveals that non-embryogenic callus can be turned into embryos and plantlets if cultured on appropriate medium. Furthermore, callus from leaf explant of C. asiatica can be a good source for production of antimicrobial compounds through bioreactor.In recent years considerable attention has been paid to utilize eco-friendly and bio-friendly plant based products, normally secondary metabolites, for the prevention and cure of different human diseases including microbial infections [1]. Moreover, the continuous and non-organized exploitation has resulted in many plants becoming rare or extinct. So there is a dire need to set a protocol for in vitro production of plants. In view of commercial importance given to secondary metabolites, efficient production of bioactive compounds by tissue culture technology has gained popularity [2]. Secondary metabolites often have a complex stereo structure and many of these cannot be synthesized economically on a commercial basis. To overcome this limitation, biotechnologist
%K Antimicrobial
%K Centella asiatica
%K organogenesis
%K plant growth regulators
%U http://www.jbioleng.org/content/5/1/13