%0 Journal Article
%T Functional cooperation of of IL-1¦Â and RGS4 in the brachial plexus avulsion mediated brain reorganization
%A Jifeng Li
%A Hui Zhao
%A Pengbo Luo
%A Yudong Gu
%J Journal of Brachial Plexus and Peripheral Nerve Injury
%D 2010
%I Thieme Medical Publishers
%R 10.1186/1749-7221-5-18
%X Adult C57BL/6 mice were divided into four groups: control, 1, 3 and 6 month of brachial plexus avulsion. IL-1¦Â, IL-6 and RGS4 expression in cortex, brainstem and spinal cord were detected by BiostarM-140 s microarray and real-time PCR. RGS4 subcellular distribution and modulation were further analyzed by primary neuron culture and Western Blot.After 1, 3 and 6 months of brachial plexus avulsion, 49 (0 up, 49 down), 29 (17 up, 12 down), 13 (9 up, 4 down) genes in cerebral cortex, 40 (8 up, 32 down), 11 (7 up, 4 down), 137 (63 up, 74 down) in brainstem, 27 (14 up, 13 down), 33 (18 up, 15 down), 60 (29 up, 31 down) in spinal cord were identified. Among the regulated gene, IL-1¦Â and IL-6 were sustainable enhanced in brain stem, while PKAC¦Â and RGS4 were up-regulated throughout cerebral cortex, brainstem and spinal cord in 3 and 6 month of nerve injury. Intriguingly, subcellular distribution of RGS4 in above three regions was dependent on the functional correlation of PKA and IL-1¦Â.Data herein indicated that brachial plexus avulsion could efficiently initiate and perpetuate the brain reorganization. Network involved IL-1¦Â and RGS4 signaling might implicate in the re-establish and strengthening of the local circuits at the cortical and subcortical levels.Neuroplasticity is the changing of neurons and the organization of their networks, which may happen through adding new cells or changing of the strength of the connections between neurons. For years it was believed that peripheral injuries could trigger a series of phenotypic changes [1,2], such as neuronal reaction and chromatolysis, even functional plasticity and brain reorganization [3,4]. It was also reported that these alterations of neural substrates occurred with time dependent manner. Namely, rapid changes within minutes are likely due to unmasking of latent synapses, while the changes over a longer time are involve many mechanisms including long-term potentiation, axonal regeneration and sprouting [5,6].Brachial
%U http://www.jbppni.com/content/5/1/18