%0 Journal Article %T EVALUATION IN SITU DE LA TOXICITE DUE AUX POLLUANTS METALLIQUES A LĄŻAIDE DĄŻUN BIOTEST BASE SUR LĄŻUTILISATION DES BACTERIES IMMOBILISEES SUR UN SUPPORT SOLIDE %A O. El HAMIANI %A H. El KHALIL %A G. BITTON %A A. BOULARBAH %J REMISE : Revue de Microbiologie Industrielle Sanitaire et Environnementale %D 2011 %I Universit¨Ś Mohammed Premier %X In order to evaluate in situ the heavy metal toxicity, research was undertaken to develop and validate the MetSTICK bioassay based on the inhibition of the activity of the -galactosidase of E. coli immobilized on a solid support specifically by heavy metals. The results showed that MetSTICK operates at a wide range of pH (4-10). The bioassay presented a high sensitivity to Hg (Minimal Inhibitory Concentration: CMI = 0.05 mg L-1), Cu (CMI = 0.3 mg L-1) and to Cd (CMI = 0.1 mg L-1) and sensitivity as the same order as the bioassay MetPAD for Zn (CMI = 0.5 mg L-1). However, MetSTICK was low sensitive for Ni (13 Keywords Olive oil mill wastewater --- Groundwater --- Sediment --- Soils --- metallic pollutants --- MetPAD --- Immobilized bacteria --- MetSTICK %K Olive oil mill wastewater %K Groundwater %K Sediment %K Soils %K metallic pollutants %K MetPAD %K Immobilized bacteria %K MetSTICK %U http://www.remise.ma/images/V2011/el%20hamianii%20et%20al.2011.pdf