%0 Journal Article
%T Synergistic Effect of Sodium Butyrate and Thalidomide in the Induction of Fetal Hemoglobin Expression in Erythroid Progenitors Derived from Cord Blood CD133 + Cells
%A Ali Dehghanifard
%A Saeid Kaviani
%A Mehrdad Noruzinia
%A Masoud Soleimani
%J Zahedan Journal of Research in Medical Sciences
%D 2012
%I Zahedan University of Medical Sciences
%X Background: The use of drugs with the ability to induce production of fetal hemoglobin as a novel therapeutic approach in treating ¦Â-Hemoglobinopathies is considered. ¦Ã-globin gene expression inducer drugs including sodium butyrate and thalidomide can reduce additional ¦Á-globin chains accumulation in erythroid precursors. Materials and Methods: In this experimental study, MACS kit was used to isolate CD133+ cells of umbilical cord blood. Further, the effect of two drugs of thalidomide and sodium butyrate were separately and combined studied on the induction of quantitative expression of ¦Â-globin and ¦Ã-globin genes in erythroid precursor cells derived from CD133+ stem cells in-vitro. For this purpose, the technique SYBR green Real-time PCR was used.Results: Flow cytometry results showed that approximately 95% of purified cells were CD133+. Real-time PCR results also showed the increased levels of ¦Ã-globin mRNA in the cell groups treated with thalidomide, sodium butyrate and combination of drugs as 2.6 and 1.2 and 3.5 times respectively, and for ¦Â-globin gene, it is respectively 1.4 and 1.3 and 1.6 times compared with the control group (p<0.05).Conclusion: The study results showed that the mentioned drug combination can act as a pharmaceutical composition affecting the induction of fetal hemoglobin expression in erythroid precursor cells derived from CD133 + cells.
%K CD133+ cells
%K ¦Ã-globin gene
%K Sodium butyrate
%K Thalidomide
%U http://www.zjrms.ir/browse.php?a_id=1317&sid=1&slc_lang=en