%0 Journal Article
%T Isolation and Characterization of Potential Phytase-Producing Fungi from Environmental Samples of Antioquia (Colombia) Aislamiento y Caracterizaci車n de Hongos Productores de Fitasa a partir de Muestras Ambientales de Antioquia (Colombia)
%A Maritza Ocampo Betancur
%A Luisa Fernanda Patiˋo Cervantes
%A Mauricio Mar赤n Montoya
%A Mauricio Salazar Yepes
%J Revista Facultad Nacional de Agronom赤a, Medell赤n
%D 2012
%I Universidad Nacional de Colombia, Sede Medell赤n
%X . Phytases are enzymes used as feed additive that enhance the phosphorus and mineral uptake in monogastric animals and reduce the level of phosphate excretion in their manure. Due to their easy cultivation and high production of extracellular enzymes, filamentous fungi are one of best sources of phytase for use in the feed industry. Phytase has been found principally in the genera Aspergillus, Penicillium, Mucor and Rhizopus. In this work, we report the isolation and characterization of environmental fungi producers of phytase with potential use as feed additives. Samples were collected from soils, fruits and cereals in Antioquia (Colombia). A total of 26 fungal strains were isolated and identified using ITS sequencing and morphological analysis. Strains belonged to the following genera: Penicillium, Aspergillus, Fusarium, Mortierella, Pestalotiopsis, Phoma, Paecilomyces and Rigidoporus. Fifty percent of isolates exhibited halos in phytase screening agar indicating that acidic phytases are common enzymes secreted by environmental fungi. Ten isolates were also able to grow in liquid phytase screening medium revealing their potential use for enzyme production in submerged fermentations. Molecular detection of the PhyA gene from Aspergillus was achieved. Partial sequence of the phyA gene from one A. niger isolate was obtained and analyzed. Resumen. Las fitasas son enzimas utilizadas como aditivo en productos de alimentaci車n animal, con el fin de mejorar la asimilaci車n de f車sforo y minerales en animales monog芍stricos y disminuir la excreci車n de f車sforo al ambiente. Los hongos filamentosos son una de las mejores fuentes de fitasas debido a su facilidad de cultivo y altos niveles de producci車n de enzimas extracelulares. Los principales productores de fitasas corresponden a miembros de los g谷neros Aspergillus, Penicillium, Mucor y Rhizopus. En este trabajo se reporta el aislamiento y caracterizaci車n de hongos ambientales productores de fitasas con aplicaci車n potencial en la industria de alimentaci車n animal. Se obtuvieron e identificaron un total de 26 aislamientos; caracterizados por secuenciaci車n de la regi車n ITS-ADNr y an芍lisis morfol車gico. Los aislamientos pertenecieron a los siguientes g谷neros: Penicillium, Aspergillus, Fusarium, Mortierella, Pestalotiopsis, Phoma, Paecilomyces y Rigidoporus. Se observ車 la secreci車n de fitasas en 50% de los aislamientos sugiriendo la ubiquidad de esta enzima en hongos ambientales. Diez aislamientos crecieron eficientemente en medio l赤quido con fitato como 迆nica fuente de f車sforo. Estos 迆ltimos cumplen con los requi
%K M谷todos moleculares
%K fosfato
%K alimento funcional
%K Aspergillus
%K Molecular methods
%K phosphate
%K functional food
%K Aspergillus
%U http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0304-28472012000100001