%0 Journal Article
%T Anti-leishmanial effect of nanosilver solutions on Leishmania tropica promastigotes by in-vitro assay
%A Ahmad Khosravi
%A Iraj Sharifi
%A Mohammad Barati
%A Mahdi Zarean
%J Zahedan Journal of Research in Medical Sciences
%D 2011
%I Zahedan University of Medical Sciences
%X Background: At present, nanosilver particles are used against a broad spectrum of microbial agents. The objective of this study was to evaluate the anti-leishmanial effect of nanosilver solutions on L. tropica promastigotes as compared with the trivalent antimony compound by colorimetric assay.Materials and Method: Promastigots of L. tropica were cultured in RPMI-1640 consisting of 10% fetal calf serum (FCS) and antibiotics at 24¡À10C. The effect of various concentrations of nanosilver solutions was evaluated in comparison with tartar emetic. Optical density as the result of tetrazolium salts (MTT) reduction by the promastigotes into a formozan product and it was calculated by Elisa reader and IC50 (50% inhibitory concentration), subsequently.Results: Both, nanosilver particles and tartar emetic inhibited the growth of L. tropica promastigotes. The IC50 for nanosilver solutions was significantly higher (14.9 ¦Ìg/mL) than the corresponding value (5.3 ¦Ìg/mL) for the control drug (p<0.001). Conclusion: Since nanosilver particles showed an effective anti-leishmanial activity, further works are required to evaluate the effect in animal model or human volunteers
%K Leishmania tropica
%K nanosilver particles
%K colorimetric assay
%U http://www.zjrms.ir/browse.php?a_id=1555&sid=1&slc_lang=en