%0 Journal Article
%T Evaluation of the magnetic field requirements for nanomagnetic gene transfection
%A A. Fouriki
%A N. Farrow
%A M.A. Clements
%A J. Dobson
%J Nano Reviews
%D 2010
%I 
%R 10.3402/nano.v1i0.5167
%X The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency. Methods: non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays. Results: Fluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well NdFeB magnet array when the magnets were 5 mm from the cell culture plate or nearer. At 6 mm and higher, fluorescence intensity decreased systematically. Conclusion: In all cases, fluorescence intensity was higher when using an oscillating array compared to a static array. For distances closer than 5 mm, the oscillating system also outperformed Lipofectamine 2000 .
%K non-viral gene delivery
%K magnetic nanoparticle-based gene transfection
%K magnetic nanoparticles
%K magnetic field
%U http://www.nano-reviews.net/index.php/nano/article/view/5167/5773