%0 Journal Article
%T Umbilical cord blood mesenchymal stem cells protect amyloid-¦Â42 neurotoxicity via paracrine
%A Ju-Yeon Kim
%A Dong Hyun Kim
%A Ji Hyun Kim
%A Yoon Sun Yang
%J World Journal of Stem Cells
%D 2012
%I Baishideng Publishing Group Co. Limited
%R 10.4252/wjsc.v4.i11.110
%X AIM: To understand the neuroprotective mechanism of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) against amyloid-¦Â42 (A¦Â42) exposed rat primary neurons. METHODS: To evaluate the neuroprotective effect of hUCB-MSCs, the cells were co-cultured with A¦Â42-exposed rat primary neuronal cells in a Transwell apparatus. To assess the involvement of soluble factors released from hUCB-MSCs in neuroprotection, an antibody-based array using co-cultured media was conducted. The neuroprotective roles of the identified hUCB-MSC proteins was assessed by treating recombinant proteins or specific small interfering RNAs (siRNAs) for each candidate protein in a co-culture system. RESULTS: The hUCB-MSCs secreted elevated levels of decorin and progranulin when co-cultured with rat primary neuronal cells exposed to A¦Â42. Treatment with recombinant decorin and progranulin protected from A¦Â42-neurotoxicity in vitro. In addition, siRNA-mediated knock-down of decorin and progranulin production in hUCB-MSCs reduced the anti-apoptotic effects of hUCB-MSC in the co-culture system. CONCLUSION: Decorin and progranulin may be involved in anti-apoptotic activity of hUCB-MSCs exposed to A¦Â42.
%K Human umbilical cord blood-derived mesenchymal stem cells
%K Decorin
%K Progranulin
%K A¦Â42
%K Anti-apoptosis
%U http://www.wjgnet.com/1948-0210/full/v4/i11/.htm