%0 Journal Article
%T Accumulation Of A Pharmacologically Important 17-Ketosteroid During Side Chain Cleavage of Cholesterol by Pseudomonas putida MTCC 1259
%A Kirti Sambhaji Pawar
%A Megha Krushnakant Bhatt
%J World Journal of Science and Technology
%D 2011
%I 
%X A screening of various microbial strains of MTCC for side chain cleavage of cholesterol was attempted. Many microorganisms were found to utilize cholesterol as sole source of carbon and energy. A pharmacologically important 17-keto steroid accumulated during side chain cleavage of cholesterol by Pseudomonas putida MTCC 1259 when n-propanol was used as an inhibitor of ring cleavage. The product accumulated during bioconversion was proposed to be 9 ¡À-hydroxy-androstenedione on the basis of Infrared and Mass Spectroscopic analysis. It is inferred that n-propanol inhibited C-1(2)-dehydrogenase of Pseudomonas putida MTCC 1259 since C-1(2)-dehydrogenated product did not appear in the bioconversion medium.
%K Cholesterol Side chain cleavage
%K Steroid bioconversion
%K Cholestenone
%K 9 ¡À-hydroxy androstadienedione
%K steroid ring cleavage inhibitors
%U http://worldjournalofscience.com/index.php/wjst/article/view/7598