%0 Journal Article
%T The effect of [Co(DH)2(An)2]2[TiF6] 3H2O coordination compound on polypeptide spectrum of amylolytic preparation from Aspergillus niger 33-19 CNMN FD 02A strain
%A Alexandra CILOCI
%A Cezara BIVOL
%A Maria STRATAN
%A Veaceslav REVA
%J Analele Universitatii din Oradea, Fascicula Biologie
%D 2012
%I University of Oradea Publishing House
%X From mycelial fungus Aspergillus niger 33-19 CNMN FD 02A cultivated on nutrient medium supplemented with [Co(DH)2(An)2]2[TiF6] 3H2O coordination compound amylolytic preparation with total ¦Á-amylase activity of 40042U/g and a specific activity of 131.3U/mg proteins was obtained. Through gel filtration and ion exchange chromatography enzyme preparation was purified 7.8 fold with a yield of 13%. SDS-PAGE electrophoresis of purified ¦Á-amylase revealed 2 polypeptide bands of 66 and 40.5kDa identified as ¦Á-amylase isomorphs similar to previously obtained results on Aspergillus niger grown without any coordination compounds. [Co(DH)2(An)2]2[TiF6] 3H2O increase the activity of non-purified amylolytic preparation, most likely due to the bonds formed between metal complex and some ¦Á-amylase radicals.
%K Aspergillus niger
%K ¦Á-amylase
%K enzyme purification
%K coordination compounds
%K SDS-PAGE
%U http://www.bioresearch.ro/bioresearch/2012-2/128-132-AUOFB.19.2.2012-CILOCI.A.-The.effect.of.[Co(DH)2(An)2]2[TiF6]_3H2O.pdf