%0 Journal Article
%T Rapid plant regeneration from nodal explants of Spilanthes acmella (L.) Murr. - an endangered medicinal plant
%A Kuldeep YADAV
%A Narender SINGH
%J Analele Universitatii din Oradea, Fascicula Biologie
%D 2012
%I University of Oradea Publishing House
%X Excised nodal explants of Spilanthes acmella (L.) Murr., ¡®Toothache Plant¡¯ proliferate rapidly in vitro on MS medium containing 0.5- 2.0 mg/l of BAP. Rapid and prolific shoot proliferation occurred. Regenerated shoots vary considerably in size (3-10 cm long) and relative stage of development, with some (50%) producing adventitious roots without transferal to a separate rooting medium. With maximum possibility of adventitious roots induction was induced from middle order nodes (3 rd to 5 th node from apex) obtained from 3 months old in vivo plant on full-strength MS medium supplemented with 1.0 mg/l BAP under the photoperiod of 18-h. The possibility of adventitious roots induction directly from regenerated shoot was greatly influenced by the concentration of BAP, photoperiod, age of donor plant and nodal position on stem.
%K In vitro propagation
%K Spilanthes acmella
%K Endangered
%K Nodal segments
%K Photoperiod
%U http://www.bioresearch.ro/bioresearch/2012-1/035-039.AUOFB.19.1.2012-YADAV.K.-Kurukshetra.Haryana.In.-Rapid.plant.regeneration.pdf